8TJT

The Fab fragment of an anti-glucagon receptor (GCGR) antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0005
Spacegroup name	H 3
Unit cell lengths	203.013, 203.013, 137.285
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.780 - 2.700
R-factor	0.2057
Rwork	0.204
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.466
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.20rc3-4406_final)
Refinement software	PHENIX (1.20rc3-4406_final)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.780	2.797
High resolution limit [Å]	2.700	2.700
Rmerge	0.088
Rmeas	0.098
Rpim	0.043
Number of reflections	57903	5827
<I/σ(I)>	17.81	2.03
Completeness [%]	99.9	99.97
Redundancy	5.2	5
CC(1/2)	0.998	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium citrate, 20 % (v/v) polypropylene glycol 3350, 0.1 M lithium chloride