8TJF
monovalent bispecific IgG antibodies through novel electrostatic steering mutations at the CH1-CL interface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9741 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.783, 78.698, 75.658 |
| Unit cell angles | 90.00, 91.84, 90.00 |
Refinement procedure
| Resolution | 37.838 - 2.300 |
| Rwork | 0.208 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.556 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.350 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.127 | |
| Number of reflections | 48672 | 3825 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.998 | 0.678 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 200 mM sodium chloride, 0.1 M Bis-Tris at pH 5.5, 25% w/v PEG 3350 |
