8TJ4

CRYSTAL STRUCTURE OF THE A/Bangkok/1/1979(H3N2) INFLUENZA VIRUS HEMAGGLUTININ WITH HUMAN RECEPTOR ANALOG 6'-SLNLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	H 3
Unit cell lengths	100.655, 100.655, 687.903
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.230 - 1.950
R-factor	0.1907
Rwork	0.189
R-free	0.22270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.957
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.230	2.030
High resolution limit [Å]	1.950	1.950
Rpim	0.070	0.470
Number of reflections	188791	21037
<I/σ(I)>	13.1	1.2
Completeness [%]	99.5	99.9
Redundancy	4.9	4.8
CC(1/2)	0.989	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	40% PEG-600 and 0.1 M CHES pH 9.5