8TCI
Crystal structure of DNMT3C-DNMT3L in complex with CGG DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-05-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.088, 189.509, 62.864 |
Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
Resolution | 44.560 - 3.190 |
R-factor | 0.2277 |
Rwork | 0.225 |
R-free | 0.25670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6u8p |
RMSD bond length | 0.003 |
RMSD bond angle | 0.562 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
High resolution limit [Å] | 3.190 | 6.890 | 3.200 |
Rmerge | 0.190 | 0.098 | 0.999 |
Rmeas | 0.224 | 0.114 | 1.192 |
Rpim | 0.118 | 0.059 | 0.645 |
Number of reflections | 23776 | 2424 | 2387 |
<I/σ(I)> | 7.2 | ||
Completeness [%] | 99.5 | 100 | 99.5 |
Redundancy | 3.4 | 3.8 | 3.3 |
CC(1/2) | 0.930 | 0.971 | 0.549 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2% v/v Tacsimate, pH 8.0, 0.1 M Tris, pH 8.5, 16% w/v PEG3350 |