8TCI

Crystal structure of DNMT3C-DNMT3L in complex with CGG DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2022-05-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	63.088, 189.509, 62.864
Unit cell angles	90.00, 90.07, 90.00

Refinement procedure

Resolution	44.560 - 3.190
R-factor	0.2277
Rwork	0.225
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6u8p
RMSD bond length	0.003
RMSD bond angle	0.562
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.190	6.890	3.200
Rmerge	0.190	0.098	0.999
Rmeas	0.224	0.114	1.192
Rpim	0.118	0.059	0.645
Number of reflections	23776	2424	2387
<I/σ(I)>	7.2
Completeness [%]	99.5	100	99.5
Redundancy	3.4	3.8	3.3
CC(1/2)	0.930	0.971	0.549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	2% v/v Tacsimate, pH 8.0, 0.1 M Tris, pH 8.5, 16% w/v PEG3350