8T70

Cryptococcus neoformans protein farnesyltransferase in complex with FPTII and TKCMIIM peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	140.768, 140.768, 129.503
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.770 - 1.892
R-factor	0.1861
Rwork	0.185
R-free	0.21180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.789
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.770	1.959
High resolution limit [Å]	1.892	1.892
Rmerge	0.117	0.688
Rmeas	0.129	0.755
Rpim	0.052	0.306
Number of reflections	103014	10141
<I/σ(I)>	8.08	1.82
Completeness [%]	99.3	98.87
Redundancy	5.7	5.9
CC(1/2)	0.992	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	290	100mM CAPSO pH9.5, 50-75mM Li2SO4, 200mM NaCl, 16%-21% PEG4000.