8T51

Crystal structure of Fab 3.10C2 bound to TREM2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 2 21 21
Unit cell lengths	66.380, 96.029, 144.316
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	1.968 - 1.900
Rwork	0.193
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.900	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.122
Number of reflections	73396	7234
<I/σ(I)>	12.9
Completeness [%]	99.9
Redundancy	6.7
CC(1/2)	0.998	0.626

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES pH6.5, 0.01 M ZnSO4, 25% PEG MME 550, 0.01 M Praseodymium acetate hydrate