8T4R

RAG2-PHD finger in complex with H3K4tBuNle peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-03-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	46.499, 46.499, 79.896
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.270 - 1.200
R-factor	0.1406
Rwork	0.140
R-free	0.16020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.153
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.270	1.240
High resolution limit [Å]	1.200	1.200
Number of reflections	31462	2806
<I/σ(I)>	31.24	0.53
Completeness [%]	98.4	86
Redundancy	29.4
CC(1/2)	1.000	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	10% PEG 5000 monomethyl ether, 0.1 M Bis-Tris, pH 6.5