8T3X

TNA polymerase, closed ternary

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-27
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	76.869, 99.525, 110.727
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.390 - 2.730
R-factor	0.247
Rwork	0.242
R-free	0.30140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.452
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.390	2.828
High resolution limit [Å]	2.730	2.730
Rmerge	0.177	0.970
Number of reflections	23008	2237
<I/σ(I)>	11.36
Completeness [%]	99.0
Redundancy	6.6
CC(1/2)		0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2 M Lithium chloride , 0.1 M HEPES pH 6.5, 17.6% PEG 6000