8T35
Crystal structure of K51 acetylated LC3A in complex with the LIR of TP53INP2/DOR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97680 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.356, 49.927, 69.784 |
Unit cell angles | 90.00, 94.61, 90.00 |
Refinement procedure
Resolution | 37.235 - 1.902 |
R-factor | 0.2292 |
Rwork | 0.228 |
R-free | 0.25880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.886 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1-2575_1496: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.235 | 1.995 |
High resolution limit [Å] | 1.902 | 1.926 |
Number of reflections | 19838 | 1751 |
<I/σ(I)> | 8.26 | |
Completeness [%] | 98.3 | |
Redundancy | 3.3 | |
CC(1/2) | 0.997 | 0.693 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG4000, 0.1M Na-citrate pH 5.5 |