8T2L

Crystal structure of LC3A in complex with the LIR of NSs4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97680
Spacegroup name	P 21 21 21
Unit cell lengths	36.995, 50.267, 137.874
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.600 - 2.240
R-factor	0.2354
Rwork	0.231
R-free	0.27620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.527
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.600	2.320
High resolution limit [Å]	2.240	2.240
Number of reflections	12899	1072
<I/σ(I)>	10.9
Completeness [%]	93.3
Redundancy	6.2
CC(1/2)	0.997	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG3350, 0.2M Na-citrate, 5% Isopropanol, 0.1M MES buffer PH 6.5