8T1G

The crystal structure of hemagglutinin form a h7n9 influenza virus (a/shanghai/1/2013) in complex with antibody 1E11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 42 21 2
Unit cell lengths	303.277, 303.277, 153.461
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.770 - 3.500
R-factor	0.1824
Rwork	0.182
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.657
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.560
High resolution limit [Å]	3.500	9.480	3.500
Rmerge	0.183	0.051	1.641
Rmeas	0.194	0.054	1.735
Rpim	0.062	0.018	0.556
Total number of observations	868574
Number of reflections	89938	4805	4457
<I/σ(I)>	3.3
Completeness [%]	99.9	99.4	100
Redundancy	9.7	8.6	9.6
CC(1/2)	0.992	0.998	0.614

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M HEPES (pH 7.5), 1.08 M sodium citrate