8SVA
Structure of the Rhodococcus sp. USK13 DarR-20 bp DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.989 |
Spacegroup name | P 65 |
Unit cell lengths | 128.894, 128.894, 113.929 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.190 - 2.960 |
R-factor | 0.2424 |
Rwork | 0.239 |
R-free | 0.27610 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 0.936 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.190 | 3.030 |
High resolution limit [Å] | 2.960 | 2.960 |
Rpim | 0.064 | 1.575 |
Number of reflections | 22523 | 1477 |
<I/σ(I)> | 8.9 | 0.5 |
Completeness [%] | 99.7 | |
Redundancy | 7.3 | |
CC(1/2) | 0.998 | 0.137 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.1 M MES pH 6.5, 30% PEG 8000 and 0.1 M Calcium acetate |