8SUV

CHIP-TPR in complex with the C-terminus of CHIC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2022-12-18
Detector	RDI CMOS_8M
Wavelength(s)	1.000020
Spacegroup name	P 1 21 1
Unit cell lengths	46.526, 82.356, 78.394
Unit cell angles	90.00, 90.03, 90.00

Refinement procedure

Resolution	46.530 - 1.630
R-factor	0.2744
Rwork	0.274
R-free	0.29030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.550
Data reduction software	XDS (0.95)
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.530	1.688
High resolution limit [Å]	1.630	1.630
Rmerge	0.149	2.287
Rmeas	0.161	2.465
Rpim	0.061	0.911
Number of reflections	71293	50246
<I/σ(I)>	8.52	0.88
Completeness [%]	96.4	94.59
Redundancy	7	7.2
CC(1/2)	0.998	0.652

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.05 M Magnesium sulfate heptahydrate, 0.1 M HEPES, 28 % v/v PEG Smear Medium