8STL
Crystal Structure of Nanobody PIK3_Nb16 against wild-type PI3Kalpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979338 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.607, 59.383, 70.702 |
| Unit cell angles | 90.00, 111.06, 90.00 |
Refinement procedure
| Resolution | 29.750 - 1.850 |
| R-factor | 0.1922 |
| Rwork | 0.189 |
| R-free | 0.25494 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7r63 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.473 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.750 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.086 | 0.530 |
| Rmeas | 0.105 | 0.694 |
| Rpim | 0.059 | 0.444 |
| Total number of observations | 113959 | 3660 |
| Number of reflections | 39095 | 1913 |
| <I/σ(I)> | 7.8 | 1.3 |
| Completeness [%] | 97.5 | |
| Redundancy | 2.9 | 1.9 |
| CC(1/2) | 0.996 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2 M Ammonium Sulfate, 0.1 M Bis-Tris pH 5.5, 25% PEG 3350 |
