8SSW

Crystal structure of DEAD-box RNA helicase DDX3X in complex with ADP at pre-unwound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 31
Unit cell lengths	68.611, 68.611, 213.878
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.750 - 2.400
R-factor	0.208
Rwork	0.207
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.515
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.127	0.061	1.205
Rpim	0.067	0.030	0.636
Number of reflections	42842	4412	4273
<I/σ(I)>	6.6
Completeness [%]	97.1	99.5	96.7
Redundancy	4.1	4.7	4.2
CC(1/2)		0.996	0.471

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	PEG3350, Magnesium Chloride, etc.