8SP7
LINE-1 retrotransposon endonuclease domain complex with tranexamic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-18 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.92 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 54.222, 64.671, 132.358 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.050 - 1.830 |
R-factor | 0.2216 |
Rwork | 0.220 |
R-free | 0.25630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.901 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.21rc1_4895) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.050 | 1.870 |
High resolution limit [Å] | 1.830 | 1.830 |
Number of reflections | 20957 | 1271 |
<I/σ(I)> | 13.9 | |
Completeness [%] | 100.0 | |
Redundancy | 13.6 | |
CC(1/2) | 0.998 | 0.897 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Tris acetate pH 6.0, 0.2 M lithium sulfate, 30% PEG 2000 monomethyl ether |