8SOZ
Structure of the complex formed by human interleukin-2 and scFv 602
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.192, 36.419, 64.189 |
| Unit cell angles | 90.00, 108.12, 90.00 |
Refinement procedure
| Resolution | 32.600 - 1.640 |
| Rwork | 0.219 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.148 |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.000 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.066 | 1.500 |
| Number of reflections | 37048 | 3555 |
| <I/σ(I)> | 5.97 | 1.01 |
| Completeness [%] | 99.4 | 96.9 |
| Redundancy | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 15-30% PEG 3350, 0.1-0.35 ammonium fluoride |
