8SHJ

Crystal structure of the WD-repeat domain of human WDR91 in complex with MR45279

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-27
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	76.859, 121.374, 131.804
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.250 - 2.210
R-factor	0.212
Rwork	0.211
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.130
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.250
High resolution limit [Å]	2.210	6.000	2.210
Rmerge	0.099	0.042	0.992
Rmeas	0.106	0.046	1.068
Rpim	0.039	0.017	0.391
Total number of observations	463068
Number of reflections	62725	3374	3080
<I/σ(I)>	5.7
Completeness [%]	99.7	99.5	99.5
Redundancy	7.4	6.8	7.3
CC(1/2)	0.996	0.999	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	12% (w/v) PEG3350, 0.2 M KCl, 0.1 M MES pH 6.5