8SE8

HTRA-1 PD/SA bound to CKP 1G10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	93
Detector technology	PIXEL
Collection date	2023-02-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	120.824, 155.362, 173.397
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 3.180
R-factor	0.2602
Rwork	0.259
R-free	0.28230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.438
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.380	3.297
High resolution limit [Å]	3.180	3.180
Rmerge	0.046	0.943
Rmeas	0.059	1.188
Rpim		0.712
Number of reflections	46183	2310
<I/σ(I)>	16.2	1.5
Completeness [%]	93.1	51.7
Redundancy	4.7	4.8
CC(1/2)	1.000	0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	15% (w/v) PEG 3350, 4% Tacsimate pH6.0 and 0.1M Mg sulfate