8S31
Crystal structure of human PLK1 Polo-Box Domain in complex with Mis18BP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.67021 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 67.147, 67.147, 254.536 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.080 - 2.134 |
R-factor | 0.1967 |
Rwork | 0.195 |
R-free | 0.22440 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.266 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.080 | 2.211 |
High resolution limit [Å] | 2.134 | 2.134 |
Rmerge | 0.104 | 0.723 |
Rmeas | 0.110 | 0.766 |
Rpim | 0.247 | |
Number of reflections | 37897 | 3442 |
<I/σ(I)> | 15.79 | 2.85 |
Completeness [%] | 99.2 | 92.13 |
Redundancy | 10 | |
CC(1/2) | 0.999 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 50 mM MES (pH 6.0), 20 mM Sodium Oxalate, 1.2 M Sodium Malonate |