8S0M
Crystal structure of the HKU1 receptor binding domain in complex with TMPRSS2 and the nanobody A01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 283 |
| Detector technology | PIXEL |
| Collection date | 2023-09-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 61 |
| Unit cell lengths | 201.870, 201.870, 210.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.970 - 3.550 |
| R-factor | 0.1943 |
| Rwork | 0.193 |
| R-free | 0.22130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.788 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.640 |
| High resolution limit [Å] | 3.550 | 3.550 |
| Rmerge | 0.265 | |
| Number of reflections | 58457 | 4358 |
| <I/σ(I)> | 14.6 | 0.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 43.3 | 41.4 |
| CC(1/2) | 1.000 | 0.223 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.35 M NaH2PO4, 0.65 M K2HPO4 |
