8RZK
The Michaelis complex of ZgGH129 D486N from Zobellia galactanivorans with neo-b/k-oligo-carrageenan tetrasaccharide (beta-kappa neo-oligo-carrageenan DP4).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9801 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 162.734, 105.943, 213.664 |
Unit cell angles | 90.00, 111.23, 90.00 |
Refinement procedure
Resolution | 47.820 - 2.290 |
R-factor | 0.1705 |
Rwork | 0.169 |
R-free | 0.20160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.810 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.890 | 2.330 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmeas | 0.161 | 1.500 |
Number of reflections | 152074 | 7468 |
<I/σ(I)> | 9.2 | 1.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.9 | 7.1 |
CC(1/2) | 0.996 | 0.551 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.63 mg/ml 2:1 ratio 0.14M Na/K-tartrate, 14% PEG 3350 |