8RU4

Crystal structure of Human Catenin Beta-1 in complex with stitched peptide inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-18
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9212
Spacegroup name	P 21 21 21
Unit cell lengths	64.567, 103.617, 187.923
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	93.961 - 2.130
Rwork	0.201
R-free	0.24030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.196
Data reduction software	DIALS
Data scaling software	STARANISO
Phasing software	MrBUMP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.961	2.310
High resolution limit [Å]	2.129	2.129
Rpim	0.036	0.319
Number of reflections	51113	2551
<I/σ(I)>	11.9	1.3
Completeness [%]	94.7	64.9
Redundancy	13.7	14.2
CC(1/2)	0.999	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	100 mM TRIS pH 8.75, 2%(w/v) PEG-6000, cryoprotected in 30% (w/v) Glucose.