8RO6
Human cohesin SMC1A-HD(longCC)/RAD21-C complex - Apo closed P-loop conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-29 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 2.075050 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.930, 64.720, 47.340 |
| Unit cell angles | 90.00, 103.07, 90.00 |
Refinement procedure
| Resolution | 46.125 - 2.200 |
| R-factor | 0.1925 |
| Rwork | 0.190 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.913 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.072 | 1.196 |
| Number of reflections | 27871 | 3967 |
| <I/σ(I)> | 12.08 | 1.05 |
| Completeness [%] | 96.2 | 87.3 |
| Redundancy | 4.9 | 4.4 |
| CC(1/2) | 0.998 | 0.513 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Hepes pH 7.0; 10% w/v PEG 6000 |
