8RD7

HUWE1 WWE domain in complex with ADP-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.999870
Spacegroup name	P 43 21 2
Unit cell lengths	62.883, 62.883, 231.245
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.460 - 1.310
R-factor	0.1491
Rwork	0.148
R-free	0.17710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	18.393
Data reduction software	XDS (Jan 31, 202)
Data scaling software	autoPROC (1.1.7)
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.679	1.437
High resolution limit [Å]	1.310	1.313
Rmerge	0.057	1.544
Number of reflections	87488	4372
<I/σ(I)>	20.5	1.5
Completeness [%]	78.3	16.7
Redundancy	12.9	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	0.1 M Na Acetate pH 4.83, 2.9 M NaCl