8RB2
The crystal structure of DNA-bound human MutSbeta (MSH2_E749A/MSH3_E976A) in the canonical mismatch bound conformation with ADP bound in MSH2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-27 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.953726 |
| Spacegroup name | P 1 |
| Unit cell lengths | 100.612, 104.290, 121.069 |
| Unit cell angles | 110.03, 91.72, 110.21 |
Refinement procedure
| Resolution | 93.250 - 2.526 |
| R-factor | 0.21123 |
| Rwork | 0.211 |
| R-free | 0.25555 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.389 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.250 | 2.570 |
| High resolution limit [Å] | 2.526 | 2.526 |
| Number of reflections | 141153 | 7027 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 98.4 | |
| Redundancy | 3 | |
| CC(1/2) | 0.997 | 0.521 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1 M MES pH 6.5 -7.5, 0.2 M Ammonium Acetate, 20 - 25 % PEG 3350 |
