8RAU
The crystal structure of DNA-bound human MutSbeta (MSH2/MSH3) in the canonical mismatch bound conformation with ADP bound in MSH2 and MSH3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99989 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.843, 91.879, 94.119 |
| Unit cell angles | 67.32, 86.93, 75.69 |
Refinement procedure
| Resolution | 46.450 - 2.330 |
| R-factor | 0.22213 |
| Rwork | 0.222 |
| R-free | 0.25753 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.374 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.450 | 2.580 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.042 | 0.441 |
| Number of reflections | 84116 | 22671 |
| <I/σ(I)> | 11.95 | |
| Completeness [%] | 94.1 | |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1 M MES pH 6.5 -7.5, 0.2 M Ammonium Acetate, 20 - 25 % PEG 3350 |
