8R67

tubulin-cryptophycin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000022
Spacegroup name	P 21 21 21
Unit cell lengths	104.750, 158.660, 180.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.520 - 2.200
R-factor	0.1953
Rwork	0.194
R-free	0.22850
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.002
RMSD bond angle	0.536
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.520	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.093	2.611
Rmeas	0.097	2.718
Rpim	0.026	0.751
Number of reflections	152380	15014
<I/σ(I)>	20.5	0.94
Completeness [%]	99.9	99.41
Redundancy	13.6	13
CC(1/2)	1.000	0.502

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	5% PEG 4K, 8% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine