8R5V
Crystal structure of bovine pancreatic ribonuclease A in complex with [Sp-PS]-mU-dT dinucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-14 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.315, 32.709, 72.802 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 17.435 - 1.800 |
| Rwork | 0.164 |
| R-free | 0.23030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.548 |
| Data reduction software | CrysalisPro (1.171.42.88a) |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | MOLREP (11.9.02) |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.435 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.078 | 0.243 |
| Rmeas | 0.094 | 0.294 |
| Rpim | 0.051 | 0.161 |
| Number of reflections | 22015 | 1248 |
| <I/σ(I)> | 9.6 | 3.4 |
| Completeness [%] | 98.8 | 95.8 |
| Redundancy | 3.1 | 2.9 |
| CC(1/2) | 0.994 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 281 | 22-25 % (w/v) PEG 4000, 20 mM sodium citrate pH 5.5 |
