8R2G
Crystal structure of a BRCA2-DMC1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 125.400, 125.400, 364.195 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 118.570 - 3.450 |
| R-factor | 0.2655 |
| Rwork | 0.264 |
| R-free | 0.30570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.480 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 182.098 | 3.692 |
| High resolution limit [Å] | 3.450 | 3.452 |
| Rmeas | 0.247 | 1.500 |
| Rpim | 0.072 | 0.561 |
| Number of reflections | 22688 | 1134 |
| <I/σ(I)> | 8 | 1.5 |
| Completeness [%] | 57.7 | 16.1 |
| Redundancy | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM HEPES-NaOH pH 7.4, 50 mM MgCl2, 500 mM NaCl, 8 % PEG 3350, 20 % glycerol |
