8R0S

Structure of reverse transcriptase from Cauliflower Mosaic Virus in complex with RNA/DNA hybrid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-08
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.032340
Spacegroup name	P 21 21 2
Unit cell lengths	88.820, 140.520, 52.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.250 - 2.350
R-factor	0.2168
Rwork	0.214
R-free	0.26310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.350	2.350
Number of reflections	28172	4464
<I/σ(I)>	10.3
Completeness [%]	99.9
Redundancy	6.7
CC(1/2)	0.990	0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	0.04M Citric acid, 0.06M Bis-Tris propane , 20% PEG 3350