8QYZ
Crystal structure of hiNES2 in complex with Xpo1 and RanGTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 97.355, 105.572, 170.400 |
| Unit cell angles | 82.08, 86.67, 76.67 |
Refinement procedure
| Resolution | 82.792 - 3.000 |
| Rwork | 0.218 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.148 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0350) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.360 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.139 | 0.995 |
| Number of reflections | 126263 | 6193 |
| <I/σ(I)> | 8.05 | 0.8 |
| Completeness [%] | 96.5 | 94.69 |
| Redundancy | 3.41 | 3.2 |
| CC(1/2) | 0.826 | 0.606 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | Tris, PEG 20K, magnesium acetate |
