8QU4
NF-YB/C Heterodimer in Complex with a 13-mer NF-YA-derived Peptide Stabilized with C8-Hydrocarbon Linker in an alternative binding pose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-07-27 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.450, 51.400, 71.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.820 - 1.380 |
R-factor | 0.1711 |
Rwork | 0.170 |
R-free | 0.19360 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.754 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.820 | 1.430 |
High resolution limit [Å] | 1.380 | 1.380 |
Rpim | 0.011 | 0.300 |
Number of reflections | 32648 | 3329 |
<I/σ(I)> | 28.52 | 1.76 |
Completeness [%] | 94.7 | 98.14 |
Redundancy | 24.5 | 20.6 |
CC(1/2) | 1.000 | 0.784 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293.15 | 100 mM Morpheus buffer system 3 pH 9, 30% Morpheus precipitant mix 1, 100 mM Morpheus Carboxylic acids |