8QN1
OPR3 variant - R283D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.070, 90.780, 111.740 |
| Unit cell angles | 90.00, 109.64, 90.00 |
Refinement procedure
| Resolution | 46.280 - 1.750 |
| R-factor | 0.2359 |
| Rwork | 0.235 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.775 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_4761: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.280 | 1.770 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 296522 | 4973 |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 97.9 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.996 | 0.725 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM Tris-HCl, 50 mM sodium tartate, 8% PEG8000 |
