8QLD
Bacteriophage T5 dUTPase mutant with loop deletion (30-35 aa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2023-09-12 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 65 |
| Unit cell lengths | 88.726, 88.726, 99.999 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.001 - 2.100 |
| Rwork | 0.221 |
| R-free | 0.26410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.342 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.094 | 0.510 |
| Number of reflections | 26147 | 2150 |
| <I/σ(I)> | 13.4 | 3.2 |
| Completeness [%] | 99.1 | |
| Redundancy | 9 | |
| CC(1/2) | 0.998 | 0.954 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | 0.085 M HEPES sodium, 1.7% v/v Polyethylene glycol 400, 1.7 M Ammonium sulfate, 15% v/v glycerol |
