8QFZ
TSLP-Bicycle complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-16 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.920, 52.349, 65.829 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.970 - 1.650 |
| R-factor | 0.18747 |
| Rwork | 0.186 |
| R-free | 0.20975 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.102 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135 STEINER) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.970 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.090 | 0.610 |
| Rmeas | 0.099 | 0.754 |
| Rpim | 0.040 | 0.429 |
| Total number of observations | 129247 | 4434 |
| Number of reflections | 23603 | 1652 |
| <I/σ(I)> | 10.1 | 1.7 |
| Completeness [%] | 93.9 | |
| Redundancy | 5.5 | 2.7 |
| CC(1/2) | 0.994 | 0.534 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% PEG MME 500/PEG 20000, 0.1 M Carboxylic acids mix and 0.1 M Buffer system 3 pH 8.5 |
