8QFB

Crystal structure of human MPP8 C-terminal region (residues 565-860)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-19
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9282
Spacegroup name	P 21 21 21
Unit cell lengths	102.556, 179.531, 228.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	93.540 - 3.040
R-factor	0.2575
Rwork	0.256
R-free	0.29470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.846
Data reduction software	xia2
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER (1.20)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	141.030	3.080
High resolution limit [Å]	3.000	3.000
Rmerge	0.081	2.360
Rmeas	0.085	2.470
Rpim	0.023	0.725
Number of reflections	85128	6191
<I/σ(I)>	14.8	1.2
Completeness [%]	100.0	100
Redundancy	13.2	11.2
CC(1/2)	0.999	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	12.5% (w/v) PEG 4000, 20% (v/v) 1,2,6-hexanetriol, 0.5 mM Morpheus II Oxometalate Mix, 0.1 M Morpheus II GlyGly/AMPD buffer mix pH 8.