8QDV
Structure of 14-3-3 zeta delta C with the bivalent tau-pS214-pS324 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972425 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.452, 59.898, 149.672 |
| Unit cell angles | 90.00, 113.18, 90.00 |
Refinement procedure
| Resolution | 137.590 - 2.500 |
| R-factor | 0.21831 |
| Rwork | 0.216 |
| R-free | 0.26001 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.127 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 137.590 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 43218 | 4507 |
| <I/σ(I)> | 8.1 | 1.3 |
| Completeness [%] | 99.6 | |
| Redundancy | 3.8 | 3.6 |
| CC(1/2) | 0.996 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M Sodium Fluoride, 0.1M bis-tris propane pH 6.5, 20% w/v PEG 3350 |
