8Q8O

PA14_16140 protein: the regulator of an operon involved in the biofilm formation in PA14 P. aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.649
Spacegroup name	P 65 2 2
Unit cell lengths	68.862, 68.862, 281.073
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.850 - 2.700
R-factor	0.2162
Rwork	0.210
R-free	0.26880
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	0.575
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.850	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.169	1.099
Rmeas	0.173	1.126
Rpim	0.037	0.243
Number of reflections	11686	1478
<I/σ(I)>	22.9	5
Completeness [%]	99.7	99.3
Redundancy	21.4
CC(1/2)	0.990	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	292	32% PEG 200, 0.2M NaCl, 0.1M acetate pH 4.5