8Q7K
IRGQ LIR2 peptide in complex with LC3B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-08-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 68.423, 68.423, 118.071 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.190 - 1.600 |
R-factor | 0.1927 |
Rwork | 0.192 |
R-free | 0.21350 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.167 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.380 | 1.657 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 37749 | 7386 |
<I/σ(I)> | 19.66 | |
Completeness [%] | 99.9 | |
Redundancy | 2 | |
CC(1/2) | 1.000 | 0.862 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 1 M lithium chloride, 0.1 M citrate, 20% PEG 6000 |