8Q5D
PfRH5 bound to monoclonal antibody MAD10-466
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-07-26 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.95374 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.464, 140.414, 83.616 |
Unit cell angles | 90.00, 112.74, 90.00 |
Refinement procedure
Resolution | 67.590 - 3.200 |
R-factor | 0.2742 |
Rwork | 0.272 |
R-free | 0.31670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.920 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4 (26-JUL-2023)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.590 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 28799 | 1404 |
<I/σ(I)> | 2.3 | |
Completeness [%] | 99.4 | |
Redundancy | 3.6 | |
CC(1/2) | 0.952 | 0.345 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Buffer System 3 pH 8.5, 0.1 M Amino Acids, 30% v/v Precipitant Mix 1 |