8Q44
Crystal structure of cA4-bound Can2 (E364R) in complex with oligo-T DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2022-05-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.714, 78.357, 94.661 |
Unit cell angles | 90.00, 95.35, 90.00 |
Refinement procedure
Resolution | 57.460 - 2.300 |
R-factor | 0.1996 |
Rwork | 0.198 |
R-free | 0.22640 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.910 |
Data reduction software | XDS (20220110) |
Data scaling software | autoPROC (20230726) |
Phasing software | PHASER (2.7.16) |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.460 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.079 | 0.771 |
Rmeas | 0.085 | 0.834 |
Rpim | 0.033 | 0.314 |
Number of reflections | 37158 | 3660 |
<I/σ(I)> | 13.22 | 2.46 |
Completeness [%] | 99.0 | 98.62 |
Redundancy | 6.8 | 6.8 |
CC(1/2) | 0.998 | 0.854 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 50 mM Tris pH 8, 17.1% (v/v) PEG 550 MME, 4.3% (w/v) PEG 2000 |