8Q2G
X-ray structure of LysECD7 endolysin against Gram-negative bacteria
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-20 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 75.568, 82.334, 64.604 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.174 - 2.490 |
Rwork | 0.268 |
R-free | 0.35800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.175 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | Arcimboldo |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.174 | 2.560 |
High resolution limit [Å] | 2.490 | 2.490 |
Number of reflections | 10864 | 3326 |
<I/σ(I)> | 13.04 | |
Completeness [%] | 97.4 | 93.83 |
Redundancy | 6 | |
CC(1/2) | 0.990 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.2 M Ammonium sulfate, 20% w/v Polyethylene glycol 3,350 pH 6.0 |