8POR

Crystal structure of wolbachia leucyl-tRNA synthetase editing domain bound to cmpd6-AMP adduct

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97924
Spacegroup name	P 61 2 2
Unit cell lengths	126.630, 126.630, 61.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.190 - 1.850
R-factor	0.14812
Rwork	0.147
R-free	0.16602
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.869
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.660	1.930
High resolution limit [Å]	1.850	1.850
Rmeas	0.090	1.890
Number of reflections	24048	1281
<I/σ(I)>	21.91	1.62
Completeness [%]	100.0	100
Redundancy	16.2	16.8
CC(1/2)	1.000	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris pH 8.5; 0.2M LiSO4; 31% w/v PEG 4000