8PNM
Structure of human KCTD15 BTB domain mutant G88D crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976254 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.262, 72.091, 94.413 |
| Unit cell angles | 89.63, 77.08, 60.50 |
Refinement procedure
| Resolution | 61.310 - 1.940 |
| R-factor | 0.238 |
| Rwork | 0.236 |
| R-free | 0.27320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.090 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.330 | 2.120 |
| High resolution limit [Å] | 1.940 | 1.960 |
| Number of reflections | 75791 | 3791 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 92.5 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.999 | 0.594 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 12.5% PEG3350, 0.2M Potassium thiocyanate |
