8PII

VHH Z70 mutant 3 in interaction with PHF6 Tau peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	P 65 2 2
Unit cell lengths	135.435, 135.435, 35.425
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.330 - 2.350
Rwork	0.200
R-free	0.26320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.390
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.330	44.330	2.430
High resolution limit [Å]	2.350	9.100	2.350
Rmerge	0.203	0.058	2.227
Rmeas	0.208	0.060	2.281
Rpim	0.045	0.013	0.490
Number of reflections	8432	187	789
<I/σ(I)>	18.8
Completeness [%]	100.0
Redundancy	38.7	29.5	40.2
CC(1/2)	0.999	0.999	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.5M ammonium sulfate, 4.5% isopropanol, 15% glycerol