8PHV

Structure of Human selenomethionylated Cdc123 bound to domain 3 of eIF2 gamma

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	60.259, 125.340, 74.081
Unit cell angles	90.00, 90.06, 90.00

Refinement procedure

Resolution	60.260 - 1.970
R-factor	0.1892
Rwork	0.186
R-free	0.22160
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.736
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.260	2.156
High resolution limit [Å]	1.970	1.974
Rmeas	0.078	1.098
Number of reflections	58305	2915
<I/σ(I)>	13.7
Completeness [%]	94.1	63.7
Redundancy	7	6.9
CC(1/2)	0.997	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% PEG3350, 8% tacsimate