8P9U
Crystal Structure of Two-Domain Laccase mutant M199A/D268N from Streptomyces griseoflavus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2021-03-18 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.302, 94.026, 118.927 |
| Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
| Resolution | 23.540 - 2.000 |
| R-factor | 0.2031 |
| Rwork | 0.201 |
| R-free | 0.24180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.895 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | MOLREP (11.6.03) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.540 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 104468 | 10454 |
| <I/σ(I)> | 8.01 | 1.43 |
| Completeness [%] | 94.6 | 95.3 |
| Redundancy | 2.9 | 2.9 |
| CC(1/2) | 0.980 | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 296 | 15 % v/v PEG Smear High, 0.15 M Ammonium acetate, 0.1 M Sodium citrate condition #31 of BCS-1 from Molecular Dimensions, UK |
