8P9C

Crystal structure of p63-p73 heterotetramer (tetramerisation domain) in complex with darpin 1810 F11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	84.024, 60.312, 53.576
Unit cell angles	90.00, 100.26, 90.00

Refinement procedure

Resolution	48.730 - 1.760
R-factor	0.17184
Rwork	0.170
R-free	0.21012
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.470
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.730	1.820
High resolution limit [Å]	1.760	1.760
Rmerge	0.049	0.392
Rmeas	0.063	0.484
Rpim	0.031	0.241
Number of reflections	25297	2481
<I/σ(I)>	12.2	2.1
Completeness [%]	96.7
Redundancy	4	4.1
CC(1/2)	0.997	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	0.2M ammonium acetate, 25% PEG3350, 0.1M HEPES pH 7.5