8P1P
USP28 in complex with AZ1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 100.444, 105.541, 178.708 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.350 - 2.760 |
R-factor | 0.2138 |
Rwork | 0.213 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.558 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.350 | 3.008 |
High resolution limit [Å] | 2.760 | 2.760 |
Number of reflections | 34461 | 1723 |
<I/σ(I)> | 10.9 | |
Completeness [%] | 94.1 | |
Redundancy | 13.3 | |
CC(1/2) | 0.998 | 0.393 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.5 M sodium malonate pH 6.0 0.1 M sodium citrate pH 5.0 |